解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The ability to prepare single-stranded chromosomal target DNA allows innovative uses of FISH technology for studies of chromosome organization. Standard FISH methodologies require functionally single-stranded DNAs in order to facilitate hybridization between the probe and the complementary chromosomal target sequence....
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000079565
更新日期:2004-01-01 00:00:00
abstract::Sperm chromosome abnormalities cut across a number of areas relevant to ICC XV. The association between increased levels of sperm aneuploidy (usually disomy) and male infertility has implications for the sessions on reproduction, sex chromosomes, aneuploidy and meiosis and was, to the best of our knowledge, first repo...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000079575
更新日期:2004-01-01 00:00:00
abstract::Mismatch repair (MMR) systems are central to maintaining genome stability in prokaryotes and eukaryotes. MMR proteins play a fundamental role in avoiding mutations, primarily by removing misincorporation errors that occur during DNA replication. MMR proteins also act during genetic recombination in steps that include ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000080593
更新日期:2004-01-01 00:00:00
abstract::ATM, the protein product of the gene mutated in the human autosomal recessive disorder ataxia telangiectasia, is involved in detection of double strand breaks (DSBs) and is a key component of the damage surveillance network of cell cycle proteins. In somatic cells ATM phosphorylates many other proteins including p53, ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000080603
更新日期:2004-01-01 00:00:00
abstract::We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. All samples were also human papilloma virus (HPV)-geno...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078559
更新日期:2004-01-01 00:00:00
abstract::To define the process of karyotypic evolution in the Galliformes on a molecular basis, we conducted genome-wide comparative chromosome painting for eight species, i.e. silver pheasant (Lophura nycthemera), Lady Amherst's pheasant (Chrysolophus amherstiae), ring-necked pheasant (Phasianus colchicus), turkey (Meleagris ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078570
更新日期:2004-01-01 00:00:00
abstract::Neuroglobin and cytoglobin are two novel members of the vertebrate globin family. Their physiological role is poorly understood, although both proteins bind oxygen reversibly and may be involved in cellular oxygen homeostasis. Here we investigate the selective constraints on coding and non-coding sequences of the neur...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078011
更新日期:2004-01-01 00:00:00
abstract::Scanning electron microscopy (SEM) proves to be an appropriate technique for imaging chromatin organization in meiosis I and II of rye (Secale cereale) down to a resolution of a few nanometers. It could be shown for the first time that organization of basic structural elements (coiled and parallel fibers, chromomeres)...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078021
更新日期:2004-01-01 00:00:00
abstract::Genetic strategies for the post-genomic sequence age will be designed to provide information about gene function in a myriad of physiological processes. Here an ENU mutagenesis program (http://reprogenomics.jax.org) is described that is generating a large resource of mutant mouse models of infertility; male and female...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078192
更新日期:2004-01-01 00:00:00
abstract::DNA methylation has been implicated in mammalian development. Transcription units contain CpG islands, but expression of CpG island associated genes in normal tissues was not believed to be controlled by DNA methylation. There are, however, numerous CpG islands containing tissue-dependent and differentially methylated...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078205
更新日期:2004-01-01 00:00:00
abstract::RNA interference (RNAi) refers to the selective degradation of mRNA induced by double-stranded RNA (dsRNA), first discovered in Caenorhabditis elegans. Homology-dependent silencing phenomena related to RNAi have been observed in many species from all eukaryotic kingdoms. RNAi and related mechanisms share several conse...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000078215
更新日期:2004-01-01 00:00:00
abstract::In animals, supernumerary chromosomes and their evolution have mostly been studied in sexual reproducing species. In the present study, for the first time, the natural distribution and stability of supernumerary microchromosomes were investigated in the unisexual fish species Poecilia formosa. Natural habitats through...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000079286
更新日期:2004-01-01 00:00:00
abstract::B chromosomes are now known in eight Brazilian rodent species: Akodon montensis, Holochilus brasiliensis, Nectomys rattus, N. squamipes, Oligoryzomys flavescens, Oryzomys angouya, Proechimys sp. 2 and Trinomys iheringi. Typically these chromosomes are heterogeneous relative to size, morphology, banding patterns, prese...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000079296
更新日期:2004-01-01 00:00:00
abstract::In rye each B chromosome (B) represents 5.5% of the diploid A genome. Rye Bs have several nuclear to whole plant effects although they seem to bear no genes except for the ones that lead to their maintenance within a population. In this context, and considering that rye Bs are enriched in repetitive non-coding regions...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000079306
更新日期:2004-01-01 00:00:00
abstract::Rye B chromosomes (Bs) have strong parasitic effects on fertility. B carrying plants are less fertile than 0B ones, whereas the Bs have no significant effects on plant vigour. On the other hand, it has been reported that B transmission is under genetic control in such a way that H line plants transmit the Bs at high f...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000079316
更新日期:2004-01-01 00:00:00
abstract::We have identified LIM-kinase (LIMK1 and LIMK2), the only known catalytic protein among LIM-family molecules. Both LIMK1 and LIMK2 phosphorylate (inactivate) cofilin, an actin depolymerizing factor, and induce actin cytoskeleton reorganization. We as well as others concurrently demonstrated that LIMK activation was re...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000076815
更新日期:2003-01-01 00:00:00
abstract::Peroxisomes are cell organelles that perform multiple functions in the metabolism of lipids and of reactive oxygen species. They are present in most eukaryotic cells. However, they are believed to be absent in spermatozoa and they have never been described in male germ cells. We have used the immortalized germ cell li...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076825
更新日期:2003-01-01 00:00:00
abstract::To obtain an estimate of the variation in common fragile sites (CFSs) among individuals, aphidicolin (APC)-induced chromosomal breakage data were analyzed for 20 karyotypically normal adult humans. As it is specifically designed to meet the analytical requirements for considering fragile sites as presence/absence char...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000073411
更新日期:2003-01-01 00:00:00
abstract::The karyotype of the pine woods treefrog, Hyla femoralis, is characterized by primitive XY female/XX male sex chromosomes. The sole difference between the X and the Y is the presence of a nucleolus organizer region (NOR) in the X. Due to a deletion of the NOR in the Y, this chromosome is distinctly smaller than the X....
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000073421
更新日期:2003-01-01 00:00:00
abstract::Translocations involving chromosomal region 19q13 are a frequent finding in follicular adenomas of the thyroid and might represent the most frequent type of structural aberration in human epithelial tumors. By positional cloning, a putative candidate gene, ZNF331 (formerly RITA) located close to the breakpoint was ide...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000074165
更新日期:2003-01-01 00:00:00
abstract::Immunolabeling using site-specific antibodies against phosphorylated histone H3 at serine 10 or serine 28 revealed in plants an almost similar temporal and spatial pattern of both post-translational modification sites at mitosis and meiosis. During the first meiotic division the entire chromosomes are highly H3 phosph...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000074175
更新日期:2003-01-01 00:00:00
abstract::We report on the conventional cytogenetic and fluorescence in situ hybridization (FISH) results obtained for a 3.5-year-old girl with developmental and language delay and a supernumerary ring chromosome mosaicism in 8% of T-lymphocytes analyzed. Using different conventional and molecular cytogenetic techniques as YAC ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076285
更新日期:2003-01-01 00:00:00
abstract::High density livestock to human comparative maps are necessary for the implementation of comparative positional candidate gene cloning. We have constructed a high-density comparative radiation hybrid (RH) map of the telomeric end of bovine chromosome 2 (BTA2) using a 12,000-rad whole genome cattle-hamster radiation hy...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076295
更新日期:2003-01-01 00:00:00
abstract::A comparative cytogenetic analysis was carried out on four Hylinae tree frogs from Brazil ((Aparasphenodon brunoi, Corythomantis greeningi, Osteocephalus langsdorffii, and Scinax fuscovarius) using Giemsa staining, BrdU replication banding, Ag-NOR staining, C-banding, DAPI and CMA(3) fluorochrome staining, and fluores...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076304
更新日期:2003-01-01 00:00:00
abstract::PCR protocols incorporating fluorescently labeled multiplexed primer combinations were developed to produce a linkage map for bison. Three hundred fifty eight microsatellite loci spanning all 29 autosomes were genotyped via 83 PCR multiplexes and nine individual amplifications. A total of 292 markers were integrated i...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000075726
更新日期:2003-01-01 00:00:00
abstract::ZOO-FISH mapping shows human chromosomes 1, 9 and 10 share regions of homology with pig chromosome 10 (SSC10). A more refined comparative map of SSC10 has been developed to help identify positional candidate genes for QTL on SSC10 from human genome sequence. Genes from relevant chromosomal regions of the public human ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000075736
更新日期:2003-01-01 00:00:00
abstract::Analysis of the horse genome is proceeding at a rapid pace. Within a short span of 6-7 years, approximately 1,500 markers have been mapped in horse, of which at least half are genes/ESTs. Health, performance and phenotypic characteristic are of major concern/interest to horse breeders and owners. Current efforts to an...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000075746
更新日期:2003-01-01 00:00:00
abstract::There is incredible morphological and behavioral diversity among the hundreds of breeds of the domestic dog, CANIS FAMILIARIS. Many of these breeds have come into existence within the last few hundred years. While there are obvious phenotypic differences among breeds, there is marked interbreed genetic homogeneity. Th...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000075756
更新日期:2003-01-01 00:00:00
abstract::Different genomic resources in chicken were integrated through the Wageningen chicken BAC library. First, a BAC anchor map was created by screening this library with two sets of markers: microsatellite markers from the consensus linkage map and markers created from BAC end sequencing in chromosome walking experiments....
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000075766
更新日期:2003-01-01 00:00:00
abstract::Gross chromosomal rearrangements and aneuploidy are among the most common somatic genomic abnormalities that occur during cancer initiation and progression, in particular in human solid tumor carcinogenesis. The loss of large chromosomal regions as consequence of gross rearrangements (e.g. deletions, monosomies, unbal...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000072844
更新日期:2003-01-01 00:00:00
abstract::To facilitate the study of the regulation and downstream interactions of genes involved in gonad development it is important to have a suitable cell culture model. We therefore aimed to characterize molecularly three different mouse gonad cell lines. TM3 and TM4 cells were originally isolated from prepubertal mouse go...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000074344
更新日期:2003-01-01 00:00:00
abstract::Spinocerebellar ataxia type 12 (SCA12), now described in European-American and Asian (Indian) pedigrees, is unique among the SCAs from clinical, pathological, and molecular perspectives. Clinically, the distinguishing feature is early and prominent action tremor with variability in other signs. Pathologically, brain M...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000072854
更新日期:2003-01-01 00:00:00
abstract::Huntington's disease (HD) is a genetically dominant neurodegenerative condition caused by an unique mutation in the disease gene huntingtin. Although the Huntington protein (Htt) is ubiquitously expressed, expansion of the polyglutamine tract in Htt leads to the progressive loss of specific neuronal subpopulations in ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000072864
更新日期:2003-01-01 00:00:00
abstract::Black porgy, Acanthopagrus schlegeli Bleeker, is a marine protandrous hermaphrodite fish. A Dmrt1 cDNA was cloned and characterized and in order to study the process of sex change in this species, mRNA transcripts of Dmrt1 were monitored. Dmrt1 was specifically transcribed in testis and seminal vesicle in 2-year-old b...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000074354
更新日期:2003-01-01 00:00:00
abstract::Members of the NFAT (nuclear factors of activated T cells) gene family have been investigated in numerous organisms, including man and mouse. All NFATs may be synthesized in several isoforms differing in amino or carboxy termini due to 5' and 3' alternative splicing of the corresponding mRNA. Recently, we mapped the m...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000066604
更新日期:2002-01-01 00:00:00
abstract::RNA-binding proteins are involved in post-transcriptional processes like mRNA stabilization, post-transcriptional modification, and transport and have been suggested to play an important role in developmental gene regulation. We report here the cloning and characterization of Brunol4, a novel mouse cDNA closely relate...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000066619
更新日期:2002-01-01 00:00:00
abstract::Holoprosencephaly (HPE) is the most common congenital malformation of the brain and face in humans. In this study we report the analysis of SIL (Sumacr;CL iumacr;nterrupting lumacr;ocus) as a candidate gene for HPE. Fluorescent in situ hybridization (FISH) analysis using a BAC 246e16 confirmed the assignment of SIL to...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000064057
更新日期:2002-01-01 00:00:00
abstract::The mouse UGRP gene family consists of two genes, Ugrp1 and Ugrp2. In this study, the genomic structure and expression patterns of Ugrp2 and its alternative spliced form were characterized. The authentic Ugrp2 gene has three exons and two introns, similar to the Ugrp1 gene, which produces a secreted protein. The Ugrp2...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000064067
更新日期:2002-01-01 00:00:00
abstract::Using FISH and RH mapping a chromosomal map of rat chromosome 10 (RNO10) was constructed. Our mapping data were complemented by other published data and the final map was compared to maps of mouse and human chromosomes. RNO10 contained segments homologous to mouse chromosomes (MMU) 11, 16 and 17, with evolutionary bre...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000063052
更新日期:2002-01-01 00:00:00
abstract::We have made a set of chromosome-specific painting probes for the American mink by degenerate oligonucleotide primed-PCR (DOP-PCR) amplification of flow-sorted chromosomes. The painting probes were used to delimit homologous chromosomal segments among human, red fox, dog, cat and eight species of the family Mustelidae...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000063032
更新日期:2002-01-01 00:00:00